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Core Facilities

The mission of the CCRTD Research Support Core Facilities is to provide the scientists of CCRTD, Clark Atlanta University, and Atlanta University Center with access to modern technology, resources, and instruments essential for cutting-edge research in molecular biology, genomics, proteomics, cell biology, and biological imaging.

CCRTD Core Laboratories are housed in the Cole Science Research Building on CAU’s campus. CCRTD Core facilities are well-equipped with modern instruments for many biological applications. Two staff scientists provide consultations and assist in application development. Additional instruments within CCRTD member laboratories are shared by all CCRTD members. For many instruments, we provide equipment maintenance, updates, purchases, and acquire basic supplies. We offer consultations on technique and proposal development and assistance with many biotechnology applications. Regularly, we invite instrument demonstrations from instrument manufacturers. CCRTD Core Facilities provide centralized supply of liquid nitrogen, pressed gasses, cubed and crushed ice, de-ionized water, and other essential supplies. We coordinate and make available to users cold storage at both 4oC, -20oC and -80oC.

CCRTD Core Laboratories received funding via by the Clark Atlanta University Research Center in Minority Institutions (RCMI) program through a grant from the National Institute on Minority and Health and Health Disparities (NIMHD) at the National Institute of Health (NIH). Currently, CCRTD core laboratories are supported by RCMI program and Georgia Research Alliance. All equipment in the CCRTD Core Facilities is available to users free of charge.

Personnel:

CCRTD Research Core Facilities employ two staff members, - a Senior Research Scientist and Facilities Laboratory Manager. Dr. Valerie Odero-Marah, Assistant Director of Research and CCRTD Research Cores, oversees the operation of CCRTD Core Facilities and is in charge of equipment acquisition and application development. Dr. Jin Zou, Senior Research Scientist, and Mr. Tony Griffin, Facilities Laboratory Manager, provide day-to-day operation of equipment and oversee user training.

 
Molecular Biology
  • -20°C Freezers (Thermo Scientific Revco Upright Freezer , and Thermo Scientific Revco Ultima Plus Chest Freezer)
  • -70°/80°C Freezers (2 Revco Chest Freezers,1 Harris Upright Freezer, 1 Thermo Scientific Revco Ultima Plus Chest Freezer, 1Thermo Scientific Value Plus Upright Freezer and 1Thermo Scientific Revco UxF Freezer)
  • Autoclaves (Steris Amsco Century SV-136H, Amsco 3023 Vacamatic Autoclave, and Tuttnauer Brinkmann 3870E Autoclave)
  • Automated X-ray processor room (AFP Mini Medical)
  • Balance ultra-sensitive (Mettler AE 100)
  • Biological Imaging and gel documentation systems (BioRad Gel Doc Molecular Imager Gel Doc XR+ w/ Image Lab Analysis Software, and Fuji Film Medical LAS 3000 w/ Image Reader 3000 Analysis Software)
  • Cell electroporator (BTX Electroporation System Electro Cell Manipulator 600)
  • Centrifuges (2 Beckman Coulter, Avanti JE w/ Rotors): Fiberlite F10BCI

            JA 25.50

            JA 20

            JA 17

            JA 14

            JA 10

            JLA 16.250

            JS 5.3

  • Deionized Water Purification System (Barnstead B-Pure and Barnstead Nano Pure Systems)
  • Freeze Dryer (Labconco FreeZone 4.5 Freeze Dry System)
  • Incubators (Fisher Scientific Isotemp 500 Series and Fisher Scientific Isotemp 655D)
  • Incubator Shakers (Small Capacity New Brunswick Scientific G24 and Large Capacity Barnstead/Labline Max Q 5000)
  • Luminometer (BMG Labtech, Lumistar Optima)
  • Microplate Spectrophotometers (BioTek Synergy H1 Multimode Hybrid Microplate Reader, and BioTek Synergy HT Microplate Reader)
  • Multimode Imager Typhoon 9410
  • PCR Machines, (2 Applied Biosystems GeneAmp PCR System 9700 w/ 96 well blocks)
  • Real-Time PCR machine (BioRad I-Cycler)
  • Real-Time PCR machine (BioRad CFX Connect System),
  • Refrigerated Circulating Waterbath (Fisher Scientific Isotemp 910)
  • Refrigerator/Freezer (Sears Kenmore)
  • Scintillation counter (Beckman Coulter LS6500 Multipurpose Scintillation Counter)
  • Sonicator (Fisher Scientific 550 Sonic Dismembrator and Misonix Sonicator 3000)
  • Spectrophotometers (Beckman DU 650, “Beckman DU 800, and Spectronic Instruments Genesys 5)
  • Speed-Vac (ThermoSavant SpeedVac SPD111V)
  • Table Top Centrifuge (Eppendorf 5810R)
  • Ultracentrifuge (Beckman Coulter Optima XPN-100, w/ Rotors):

                  SW 65

                  SW 55 TI 

                  SW 50.1

                  SW 40 TI

                  SW 32 TI

                  Type70.1TI

                  Type 65

                  Type 45 TI

                  Type 40

  • UV Stratalinker (UVP CL 1000)
  • Vacuum Oven (Fisher Scientific Isotemp Vacuum Oven Model 282A)
 
Cell Biology
  • Air Jacketed, Self Decontaminating, with Copper Interior Carbon Dioxide Incubators (4 Nuaire IR C3)
  • Biological Safety Cabinets (2 Labconco Purifier Logic Biological Safety Cabinets),
  • Cell Counter (Nexcelom Cellometer Vision)
  • Cryopreservation tanks with monitored liquid nitrogen supply (2 Thermolyne Locator Jr. Cryo Biological System Storage, Cryosafe Cryo Biological System Storage, Custom Biogenics Systems Cryosystems 6001 Storage and 2 Thermo Scientific Locator 4 Cryo Biological System Storage)
  • Deionized Water Purification System (Barnstead B-Pure and Barnstead Nano Pure Systems)
  • Inverted Digitized Microscope (Zeiss Axiovert 200 Inverted Microscope)
  • Inverted Manual Microscope (Fisher Scientific)
  • Fluorescence-Activated Cell Sorter (Beckton Dickinson Biosciences FACSJazz Cell Sorter)
  • Fluorescence Enabled Inverted Digitized Microscope with live cell imaging modules (Zeiss Axiovert 200 Inverted Microscope)
  • Fluorescence Enabled Upright Digitized Microscope with Apotome (Zeiss Imager Z1Upright Microscope)
  • Confocal Fluorescent Microscope (Zeiss Observer Z1 Microscope with LSM 700 Confocal Imaging)
  • Flow Cytometer (Accuri C6)
  • Refrigerators/Freezers (Sears Kenmore)
  • Tabletop Centrifuges (1 Beckman GS-6R, and 2 Eppendorf 5702 Centrifuges)
  • Water-baths (Fisher Scientific Isotemp 215 and Fisher Scientific Isotemp 220)
  • Water Jacketed Carbon Dioxide Incubators (4 Nuaire IR Autoflow Incubators)
 

Cancer Genomics and Bioinformatics

  • -20°C Freezers (Thermo Scientific Revco Upright Freezer)
  • Bioanalyzer (Agilent Technologies Bioanalyzer 2100)
  • Centrifuge (Eppendorf 5417R)
  • Coldbox Refrigerator (Symphony)
  • Covaris Instrument/Sonicator (Covaris Inc.)
  • DNA Hydroshear (Digilabs)
  • DNA Sequencer (Applied Biosystems SOLiD 4 System Sequencer)
  • EZ Bead System (Applied Biosystems EZ Bead Amplifier, Emulsifier, and Enricher)
  • Nano Drop Spectrophotometer (Thermo Scientific Nano Drop 2000C)
  • PCR Machine (Applied Biosystems GeneAmp PCR System 9700 w/ 96 well blocks)
  • Recirculator Bath (VWR International)
  • Server Cluster (Penquin Entry Level Computing Cluster)
  • Computer Workstation Dell (Dell Optiplex GX620)
 

Proteomics

  • Fast Protein Liquid Chromatography System (FPLC)
  • High Pressure Liquid Chromatography System (HPLC) (Shimadzu UFLC)
  • Centrivap Concentrator with Cold Trap (Labconco)
  • High-speed tabletop ultra-centrifuge (Beckman Optima TL Ultracentifuge)
  • Refrigerator/Freezer (Roper)
 

Histology

  • Autoclave for antigen retrieval (Tuttnauer Brinkmann)
  • Auto Stainer (Dako Autostainer Link 48)
  • Axio Imager Z.1 microscope (Zeiss)
  • Binoculars (Wild Heerbrurgg Switzerland, American Optical Corporation Model 569)
  • Compound Microscope (Am Scope)
  • Compound Microscope (Bausch & Lomb)
  • Cryostat (Thermo Scientific HM 525)
  • Dissection Microscope (American Optical Corporation)
  • Embedding and slicing equipment (LKB Bromma 7800 Knifemaker, RM Glass Knifemaker)
  • Freezer/Refrigerator (VWR International)
  • Fume hood (Hamilton Safeaire VAV)
  • Galaxy mini table top centrifuge (VWR International)
  • Laser Capture Microdisection Microscope (Leica LMD 6000)
  • LMD CTR 6500 Laser Micro dissection (Leica)
  • M-3000 Compound Microscope (Baxter)
  • Microtome HM 310 (Thermo Scientific)
  • 820 Spencer Microtome (American Optical Corporation)
  • Scout Pro Digital Balance (Ohaus)
  • Slide mounting (Clinical Scientific Equipment Company Slide Warmer No. 26020)
  • Slide Staining Station (Thermo Scientific)
  • Slide Warmer (Clinical Scientific Equipment)
  • Standard Analog Orbital Shaker (VWR International)
  • Thelco Constant temperature incubator (Precision Scientific)
  • Tissue Floatation Bath (Thermo Scientific)
  • Tissue/Cell Imaging System (Leica Aperio Versa 8)
  • Ultramicrotome with diamond knife (Sorvall Porter-Blum Ultra-Microtome MT-2)
  • Wax Block Cooler (Thermo Scientific)
  • Wax Block Storage 10 tray organizer (Thermo Scientific)
  • Wax Dispenser (Thermo Scientific)
 

Structural Biology

  • Inverted digitized microscope (Zeiss Axiovert 200 Inverted Microscope)
  • Fluorescence-enabled inverted digitized microscope with Z-stacking and live cell imaging modules (Zeiss
  • Axiovert Inverted microscope)
  • Fluorescence Enabled Upright Digitized Microscope with Apotome (Zeiss Imager Z1Upright Microscope)
  • Confocal Fluorescent Microscope (Zeiss Observer Z1 Microscope with LSM 700 Confocal Imaging)
 

Tissue Repository

  • Liquid Nitrogen Tissue Storage (Custom Biogenics Systems Cryosystems 6001 Storage)
  • Isothermal Freezers (2 Custom Biogenic Systems V1500 AB Series)
  • -70°/80°C Freezer (Thermo Scientific Revco UxF)

      Supplies

  • Purified water for biochemical and molecular biology applications. Core Facilities maintains two water purification systems and provides clean water of two grades (reagent grade and ultrapure grade) to all users
  • Liquid nitrogen
  • Carbon dioxide
  • Ice, crushed