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CENTER FOR CANCER RESEARCH AND THERAPEUTIC DEVELOPMENT

Protemics

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We are equipped for proteomics research including Cell Fractionation on one floor and tabletopUltracentrifuges (Beckman Coulter) , Fast Protein Liquid Chromatography (FPLC), Two-Dimensional Protein Electrophoresis (Protean II, Biorad), and Gel Documentation Systems (one Bio-Rad and one Fuji Medical). Protein quantification can be done using UV-Vis spectrophotometry ( DU650, Beckman Coulter) or multimode Plate Spectrophotometers in both 96-well and 384-well micro well plate formats. Protein gels can also be visualized, digitized, and documented on Multi-Mode ImagerTyphoon 9410.

List of equipment for proteomics research in CCRTD:

Isoelectrofocusing unit with high-voltage power supply (Bio-Rad) 
Fast Protein Liquid Chromatography System (FPLC) 
High-speed tabletop ultra-centrifuge (Beckman Coulter) 
NMR facility (supported by Chemistry Department) 
Plate spectrophotometer with UV-Vis, Fluorescence, TOF, and luminescence detection modes in 96 and 384-well formats (BioTek) 
Two-dimensional protein separation setup including accessories (Protean II, Bio-Rad)